Protocols and Tools
Proteomics Protocols
- General sample preparation guide
- Large-scale immunoprecipitation protocol
- Desalting proteins and peptides using chloroform-methanol extraction
- C18 Sep-Pak purification of peptides
- In-gel digestion protocol
- In-solution digestion protocol
- Silver Stain Protocol
- Zip-tip purification and spotting on MALDI target
- ClinProt protocols for MALDI
- TCA/DOC protein precipitation protocol
Proteomics Tools
- CPF Proteomics Pipeline
- Proteomics: In house Mascot Server
- Matrixscience: Mascot
- Proteomics Server: Expasy
- Protein Prospector
- Merops protease database
- Peptide Property Calculator
- MS/MS Fragment Ion Calculator
- LIPID MAPS (Search with masses)
- Web-based calculator for ROC curves
- GGBase - Database for protein ubiquitylation
- Phosida - Database for protein phosphorylation, acetylation and glycosylation