Automated Image Analysis

Automated Analysis of High Content Imaging Data

Segment

The Operetta system comes with Harmony software which allows automated analysis of image data. An example of the automated analysis is shown in the picture (provided by Dr Matt Benson, Bhattacharya Group), where cells stained with DAPI, Mitotracker and Lysotracker were imaged, and then analysed to find and segment the nuclei, cytoplasm, exclude border cells, and then identify and segment the lysosomes. This allows quantitative measurements of organelle characteristics.

Contact Details

Professor Shoumo Bhattacharya MD MSc (Biochem) FRCP FESC FMedSci

BHF Chair of Cardiovascular Medicine
Department of Cardiovascular Medicine & Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford
OX3 7BN

Email: shoumo.bhattacharya@well.ox.ac.uk
PA: Mrs Jan Duff
Email: jan.duff@well.ox.ac.uk

British Heart Foundation

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