Metabolomics & Lipidomics

metabolomics

In the group, we have the expertise to measure a range of metabolites by using either LC-MS or GCxGC-MS based analytical platforms. From samples such as cell extracts, tissue extracts, blood plasma/serum or urine, we can measure amino acids, fatty acids, lipid subsets, cholesterol and derivatives, nucleosides and small polar components of glycolysis and the Krebs cycle including lactate.

For enquiries, please contact Prof. Benedikt Kessler, Dr. Zhanru Yu (zhanru.yu@ndm.ox.ac.uk), Dr. Honglei Huang (honglei.huang@ndm.ox.ac.uk)

 

Selected References:

Mills EL, Ryan DG, Prag HA, Dikovskaya D, Menon D, Zaslona Z, Jedrychowski MP, Costa ASH, Higgins M, Hams E, Szpyt J, Runtsch MC, King MS, McGouran JF, Fischer R, Kessler BM, McGettrick AF, Hughes MM, Carroll RG, Booty LM, Knatko EV, Meakin PJ, Ashford MLJ, Modis LK, Brunori G, Sévin DC, Fallon PG, Caldwell ST, Kunji ERS, Chouchani ET, Frezza C, Dinkova-Kostova AT, Hartley RC, Murphy MP, O'Neill LA. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature. 2018 Mar 28. doi: 10.1038/nature25986. Read more

Riffelmacher T, Clarke A, Richter FC, Stranks A, Pandey S, Danielli S, Hublitz P, Yu Z, Johnson E, Schwerd T, McCullagh J, Uhlig H, Jacobsen SEW, Simon AK. Autophagy-Dependent Generation of Free Fatty Acids Is Critical for Normal Neutrophil Differentiation. Immunity. 2017 Sep 19;47(3):466-480.e5. doi: 10.1016/j.immuni.2017.08.005. READ MORE

Yu Z, Huang H, Reim A, Charles PD, Northage A, Jackson D, Parry I, Kessler BM. 2017. Optimizing 2D gas chromatography mass spectrometry for robust tissue, serum and urine metabolite profiling. Talanta, 165 pp. 685-691. | Show Abstract | Read more

Zauri M, Berridge G, Thézénas ML, Pugh KM, Goldin R, Kessler BM, Kriaucionis S. 2015. CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer. Nature, 524 (7563), Read abstract | Read more

Akhtar MZ, Huang H, Kaisar M, Lo Faro ML, Robolledo R, Morten K, Heather LC, Dona A et al. 2015. Using an integrated -omics approach to identify key cellular processes that are disturbed in the kidney following brain death. Am J Transplant, Read abstract | Read more

Valli A, Rodriguez M, Moutsianas L, Fischer R, Fedele V, Huang HL, Van Stiphout R, Jones D et al. 2015. Hypoxia induces a lipogenic cancer cell phenotype via HIF1α-dependent and -independent pathways. Oncotarget, 6 (4), Read abstract | Read more

Dulneva A, Lee S, Oliver PL, Di Gleria K, Kessler BM, Davies KE, Becker EB. 2015. The mutant Moonwalker TRPC3 channel links calcium signaling to lipid metabolism in the developing cerebellum. Hum Mol Genet, 24 (14), Read abstract | Read more

Huang H, Akhtar MZ, Ploeg RJ, Kessler BM. 2014. Lipidomics Techniques and its Applications in Medical Research Journal of Glycomics & Lipidomics, 04 (02), | Read more