Phosphoproteomics

 

 

phophoproteomics

Within the group and the Advanced Proteomics Facility, we have experience with mapping protein phosphorylation sites on individual proteins, but also on a global level. The latter approach uses hydrophilic interaction chromatography (HILIC) combined with ion metal affinity chromatography (IMAC) for efficient enrichment of phosphorylated peptides.

For further inquiries, please contact Dr. Roman Fischer or Prof. Benedikt Kessler

 

 

 

Selected References

Raducu M, Fung E, Serres S, Infante P, Barberis A, Fischer R, Bristow C, Thézénas ML, Finta C, Christianson JC, Buffa FM, Kessler BM, Sibson NR, Di Marcotullio L, Toftgård R, D'Angiolella V. SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development. EMBO J. 2016 Jul 1;35(13):1400-16. doi: 10.15252/embj.201593374. Read more

LOCHMATTER C, FISCHER R, CHARLES PD, POWRIE F, KESSLER BM. Integrative Phosphoproteomics Links IL-23R Signaling with Metabolic Adaptation in Lymphocytes. Sci Rep. 2016 Apr 15;6:24491. doi: 10.1038/srep24491. Read more

Mathea S, Abdul Azeez KR, Salah E, Tallant C, Wolfreys F, Konietzny R, Fischer R, Lou HJ et al. 2016. Structure of the Human Protein Kinase ZAK in Complex with Vemurafenib. ACS Chem Biol, Read abstract | Read more

Maniam S, Coutts AS, Stratford MR, McGouran J, Kessler B, La Thangue NB. 2015. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase. Cell Death Differ, 22 (1), Read abstract | Read more

Parsons JL, Khoronenkova SV, Dianova II, Ternette N, Kessler BM, Datta PK, Dianov GL. 2012. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress. Nucleic Acids Res, 40 (22), Read abstract | Read more

Carr SM, Munro S, Kessler B, Oppermann U, La Thangue NB. 2011. Interplay between lysine methylation and Cdk phosphorylation in growth control by the retinoblastoma protein. EMBO J, 30 (2), Read abstract | Read more

Fox JL, Ismail F, Azad A, Ternette N, Leverrier S, Edelmann MJ, Kessler BM, Leigh IM, Jackson S, Storey A. 2010. Tyrosine dephosphorylation is required for Bak activation in apoptosis. EMBO J, 29 (22), Read abstract | Read more

Edelmann MJ, Kramer HB, Altun M, Kessler BM. 2010. Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion. FEBS J, 277 (11), Read abstract | Read more