Within the group and the Advanced Proteomics Facility, we have experience with mapping protein phosphorylation sites on individual proteins, but also on a global level. The latter approach uses hydrophilic interaction chromatography (HILIC) combined with ion metal affinity chromatography (IMAC) for efficient enrichment of phosphorylated peptides.
For further inquiries, please contact Dr. Roman Fischer or Prof. Benedikt Kessler
Raducu M, Fung E, Serres S, Infante P, Barberis A, Fischer R, Bristow C, Thézénas ML, Finta C, Christianson JC, Buffa FM, Kessler BM, Sibson NR, Di Marcotullio L, Toftgård R, D'Angiolella V. SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development. EMBO J. 2016 Jul 1;35(13):1400-16. doi: 10.15252/embj.201593374. Read more
LOCHMATTER C, FISCHER R, CHARLES PD, POWRIE F, KESSLER BM. Integrative Phosphoproteomics Links IL-23R Signaling with Metabolic Adaptation in Lymphocytes. Sci Rep. 2016 Apr 15;6:24491. doi: 10.1038/srep24491. Read more
ACS Chem Biol, Read abstract | Read more
2016. Structure of the Human Protein Kinase ZAK in Complex with Vemurafenib.
Cell Death Differ, 22 (1), Read abstract | Read more
2015. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
Nucleic Acids Res, 40 (22), Read abstract | Read more
2012. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress.
EMBO J, 30 (2), Read abstract | Read more
2011. Interplay between lysine methylation and Cdk phosphorylation in growth control by the retinoblastoma protein.
EMBO J, 29 (22), Read abstract | Read more
2010. Tyrosine dephosphorylation is required for Bak activation in apoptosis.
FEBS J, 277 (11), Read abstract | Read more
2010. Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion.