Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The initiation of most cytotoxic immune responses requires MHC class I-restricted presentation of internalized antigens to CD8(+) T lymphocytes, a process called cross-presentation. In dendritic cells (DC), the only antigen-presenting cells that activate naive T cells, cross-presentation is particularly efficient after internalization of opsonized antigens or immune complexes, which are cross-presented through a proteasome- and transporter associated with antigen processing (TAP)-dependent MHC class I antigen presentation pathway. We now show that FcgammaR-mediated cross-presentation is tightly regulated during DC maturation. Cross-presentation increases soon after activation by lipopolysaccharides, and it is then inhibited in fully mature cells. The initial induction of cross-presentation results from an increase of both antigen internalization and delivery to the cytosol, and from a slight rise in the activity of the proteasome and TAP. The subsequent block of cross-presentation in mature DC is a consequence of the selective down-modulation of antigen internalization and cytosolic delivery, while proteasome and TAP activities continue to rise. Therefore, FcgammaR-mediated cross-presentation is regulated during DC maturation by the selective control of antigen internalization and transport to the cytosol.

Original publication

DOI

10.1002/eji.200324508

Type

Journal article

Journal

Eur J Immunol

Publication Date

02/2004

Volume

34

Pages

398 - 407

Keywords

ATP-Binding Cassette Transporters, ATP-Binding Cassette, Sub-Family B, Member 3, Animals, Antigen Presentation, Antigen-Antibody Complex, Cell Differentiation, Cysteine Endopeptidases, Dendritic Cells, Egg Proteins, Flow Cytometry, Histocompatibility Antigens Class I, Immunophenotyping, Mice, Mice, Inbred C57BL, Mice, Knockout, Multienzyme Complexes, Ovalbumin, Peptide Fragments, Proteasome Endopeptidase Complex