Phosphoproteomics
Within the group and the Advanced Proteomics Facility, we have experience with mapping protein phosphorylation sites on individual proteins, but also on a global level. The latter approach uses hydrophilic interaction chromatography (HILIC) combined with ion metal affinity chromatography (IMAC) for efficient enrichment of phosphorylated peptides.
For further inquiries, please contact Dr. Roman Fischer or Prof. Benedikt Kessler
Selected References
Raducu M, Fung E, Serres S, Infante P, Barberis A, Fischer R, Bristow C, Thézénas ML, Finta C, Christianson JC, Buffa FM, Kessler BM, Sibson NR, Di Marcotullio L, Toftgård R, D'Angiolella V. SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development. EMBO J. 2016 Jul 1;35(13):1400-16. doi: 10.15252/embj.201593374. Read more
LOCHMATTER C, FISCHER R, CHARLES PD, POWRIE F, KESSLER BM. Integrative Phosphoproteomics Links IL-23R Signaling with Metabolic Adaptation in Lymphocytes. Sci Rep. 2016 Apr 15;6:24491. doi: 10.1038/srep24491. Read more
MATHEA S, ABDUL AZEEZ KR, SALAH E, TALLANT C, WOLFREYS F, KONIETZNY R, FISCHER R, LOU HJ, BRENNAN PE, SCHNAPP G, PAUTSCH A, KESSLER BM, TURK BE, KNAPP S . 2016. Structure of the Human Protein Kinase ZAK in Complex with Vemurafenib. ACS Chem Biol, Read abstract | Read more
MANIAM S, COUTTS AS, STRATFORD MR, MCGOURAN J, KESSLER B, LA THANGUE NB. 2015. Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase. Cell Death Differ, 22 (1), Read abstract | Read more
PARSONS JL, KHORONENKOVA SV, DIANOVA II, TERNETTE N, KESSLER BM, DATTA PK, DIANOV GL.2012. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress. Nucleic Acids Res, 40 (22), Read abstract | Read more
CARR SM, MUNRO S, KESSLER B, OPPERMANN U, LA THANGUE NB. 2011. Interplay between lysine methylation and Cdk phosphorylation in growth control by the retinoblastoma protein. EMBO J, 30 (2), Read abstract | Read more
FOX JL, ISMAIL F, AZAD A, TERNETTE N, LEVERRIER S, EDELMANN MJ, KESSLER BM, LEIGH IM, JACKSON S, STOREY A. 2010. Tyrosine dephosphorylation is required for Bak activation in apoptosis. EMBO J, 29 (22), Read abstract | Read more
EDELMANN MJ, KRAMER HB, ALTUN M, KESSLER BM. 2010. Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion. FEBS J, 277 (11), Read abstract | Read more