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Tri-functional activity-based protein probes that encompass an electrophilic trap, a photo-reactive group and a bio-orthogonal ligation handle are described. With these, and in a three-step chemical proteomics approach, proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger-Bertozzi ligation for visualization and identification of the resulting conjugates.

Original publication

DOI

10.1039/c0cc03322g

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

21/12/2010

Volume

46

Pages

9052 - 9054

Keywords

Animals, Catalytic Domain, Cell Line, Tumor, Mice, Molecular Probes, Photochemical Processes, Proteasome Endopeptidase Complex