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The transcription factor HIF (hypoxia-inducible factor) mediates a highly pleiotrophic response to hypoxia. Many recent studies have focused on defining the extent of this transcriptional response. In the present study we have analysed regulation by hypoxia among transcripts encoding human Fe(II)- and 2-oxoglutarate-dependent oxygenases. Our results show that many of these genes are regulated by hypoxia and define two groups of histone demethylases as new classes of hypoxia-regulated genes. Patterns of induction were consistent across a range of cell lines with JMJD1A (where JMJD is Jumonji-domain containing) and JMJD2B demonstrating robust, and JMJD2C more modest, up-regulation by hypoxia. Functional genetic and chromatin immunoprecipitation studies demonstrated the importance of HIF-1alpha in mediating these responses. Given the importance of histone methylation status in defining patterns of gene expression under different physiological and pathophysiological conditions, these findings predict a role for the HIF system in epigenetic regulation.

Original publication

DOI

10.1042/BJ20081238

Type

Journal article

Journal

Biochem J

Publication Date

15/12/2008

Volume

416

Pages

387 - 394

Keywords

Amino Acids, Dicarboxylic, Cell Line, Deferoxamine, Gene Expression Profiling, Gene Expression Regulation, Enzymologic, Humans, Hypoxia, Hypoxia-Inducible Factor 1, alpha Subunit, Jumonji Domain-Containing Histone Demethylases, Ketoglutaric Acids, Molecular Sequence Data, Neoplasm Proteins, Oligonucleotide Array Sequence Analysis, Oxidoreductases, N-Demethylating, Oxygenases, Promoter Regions, Genetic, Protein Subunits, RNA, Small Interfering, Siderophores, Transcription Factors