Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We describe a "gel-assisted" proteomic sample preparation method for mass spectrometry analysis. Solubilized protein extracts or intact cells are co-polymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation and matrix formation. Gel-aided sample preparation (GASP) has been optimized to be highly flexible, scalable and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types and accessible to non-specialists.

Original publication




Journal article




Wiley-VCH Verlag

Publication Date