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We describe a "gel-assisted" proteomic sample preparation method for mass spectrometry analysis. Solubilized protein extracts or intact cells are co-polymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation and matrix formation. Gel-aided sample preparation (GASP) has been optimized to be highly flexible, scalable and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types and accessible to non-specialists.

Original publication

DOI

10.1002/pmic.201400436

Type

Journal article

Journal

Proteomics

Publisher

Wiley-VCH Verlag

Publication Date

17/12/2014