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Interactions between biological pathways and molecular oxygen require robust mechanisms for detecting and responding to changes in cellular oxygen availability, to support oxygen homeostasis. Peptidylglycine α-amidating monooxygenase (PAM) catalyzes a two-step reaction resulting in the C-terminal amidation of peptides, a process important for their stability and biological activity. Here we show that in human, mouse, and insect cells, peptide amidation is exquisitely sensitive to hypoxia. Different amidation events on chromogranin A, and on peptides processed from proopiomelanocortin, manifest similar striking sensitivity to hypoxia in a range of neuroendocrine cells, being progressively inhibited from mild (7% O2) to severe (1% O2) hypoxia. In developing Drosophila melanogaster larvae, FMRF amidation in thoracic ventral (Tv) neurons is strikingly suppressed by hypoxia. Our findings have thus defined a novel monooxygenase-based oxygen sensing mechanism that has the capacity to signal changes in oxygen availability to peptidergic pathways.

Original publication

DOI

10.1074/jbc.M115.667246

Type

Journal article

Journal

J Biol Chem

Publication Date

09/10/2015

Volume

290

Pages

24891 - 24901

Keywords

copper monooxygenase, hypoxia, hypoxia-inducible factor (HIF), peptide hormone, secretion, Amides, Amino Acid Sequence, Animals, Cell Hypoxia, Cell Line, Chromogranin A, Drosophila melanogaster, Humans, Mice, Mixed Function Oxygenases, Molecular Sequence Data, Multienzyme Complexes, Neuroendocrine Cells, Oxygen