Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles
Journal article
Céspedes PF. et al, (2022), Nature Communications, 13
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Hermansky-Pudlak syndrome type 1 causes impaired anti-microbial immunity and inflammation due to dysregulated immunometabolism.
Journal article
Cavounidis A. et al, (2022), Mucosal immunology
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Biochemical and metabolic maladaption defines pathological niches in progressive multiple sclerosis
Preprint
Grant-Peters M. et al, (2022)
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LARP1 regulates metabolism and mTORC1 activity in cancer
Preprint
Chettle J. et al, (2022)
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Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation
Journal article
Zhou D. et al, (2022), Nature Communications, 13