Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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CSF extracellular vesicle proteomics demonstrates altered protein homeostasis in amyotrophic lateral sclerosis
Journal article
Thompson AG. et al, (2020), Clinical Proteomics, 17
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TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts
Journal article
Fielden J. et al, (2020), Nature Communications, 11
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An ALS-linked mutation in TDP-43 disrupts normal protein interactions in the motor neuron response to oxidative stress
Journal article
Feneberg E. et al, (2020), Neurobiology of Disease, 144, 105050 - 105050
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Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.
Journal article
van de Weijer ML. et al, (2020), Molecular cell, 80, 374 - 375
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Glycosylated Siglec-6 expression in syncytiotrophoblast-derived extracellular vesicles from preeclampsia placentas.
Journal article
Awoyemi T. et al, (2020), Biochemical and biophysical research communications