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This transfection reagent panel was assembled by the Target Discovery Institute HTS Facility as a representative sample of the siRNA transfection reagents currently available. This list is not exhaustive and additional transfection reagents specially formulated for particular cell types are available through numerous smaller vendors.

We suggest the first step of any transfection optimization include a thorough literature search followed by consultation with a member of the TDI HTS facility to narrow the field before experiments begin.

Samples of most of these transfection reagents listed below are available for optimization in the TDI HTS facility. Please contact us when you are beginning your transfection optimization.

The links below provide additional details about each of the reagents available from vendors along with protocols detailing their use.

Transfection reagents highlighted in BOLD in the list are held as part of the TDI stock transfection reagent panel. These transfection reagents have been tested across a range of cell types at the TDI.

Transfection Reagent Link to Protocol(s)

Invitrogen (Life Technologies)

http://www.invitrogen.com/site/us/en/home.html
Lipofectamine®
Lipofectamine® 2000
Lipofectamine® LTX
Lipofectamine® RNAiMAX
FreeStyleTM MAX Reagent
293FectinTM Transfection Reagent
OligofectamineTM Transfection Reagent
DMRIE-C Transfection Reagent
OptifectTM Transfection Reagent (Transfection of low confluence cells)
Lipofectin® Transfection Reagent
siPORTTM Amine Transfection Agent
siPORTTM NeoFXTM Transfection Agent

Lipofectamine Reagent
Lipofectamine 2000 Reagent
Lipofectamine LTX and PLUS Reagents
Lipofectamine RNAiMAX
FreeStyle MAX Reagent
293fectin Transfection Reagent
Oligofectamine Reagent
DMRIE-C
Optifect Reagent

Lipofectin Reagent
Protocol: siPORT Amine Transfection Agent
siPORTTM NeoFXTM Transfection Agent Protocol

Dharmacon (Thermo) http://www.dharmacon.com/Home.aspx?id=642
Dharamfect 1
Dharmafect 2
Dharmafect 3
Dharmafect 4
basic-dharmafect-protocol(1).pdf
Biontex http://www.biontex.com/
DOTAP
Metafectene
Metafectene Pro
Metafectene SI
Metafectene Easy +

DOTAP Protocol
Metafectene Protocol
Metafectene Pro Protocol
Metafectene SI
Metafectene Easy + Protocol

Genlantis http://www.genlantis.com/
GeneSilencer
GenePORTER
GenePORTER 2
GenePORTER 3000
GenePORTER Gold
GenePORTER H
PerFectin

GeneSilencer Protocol
GenePORTER
GenePORTER 2
GenePORTER 3000
GenePORTER Gold
GenePORTER H

PerFectin

Mirus http://www.mirusbio.com/
TransIT®-2020 Reagent
TransIT®-LT1 Reagent
TransIT®-Express Reagent
TransIT-TKO® Reagent
TransIT-siQUEST® Reagent
TransIT-2020 Reagent Protocol
TransIT-LT1 Reagent Protocol
TransIT-Express Reagent Protocol
TransIT-TKO Reagent Protocol
TransIT-siQUEST Reagent Protocol
New England BioLabs (NEB) https://www.neb.com/
TransPass HUVEC Transfection Reagent
TransPass R2 Transfection Reagent
TransPass D1 Transfection Reagent
TransPass D2 Transfection Reagent
TransPass V
TransPass HUVEC Protocol
TransPass R2 Protocol
TransPass D1 Protocol
TransPass D2 Protocol
TransPass V Protocol
Merck http://www.merck-chemicals.co.uk/
293-FreeTM Transfection Reagent (HEK 293 suspension cultures)
NanoJuice® Transfection Kit
ProteoJuiceTM Protein Transfection Reagent (transfection of intact functional protein)
RiboJuiceTM siRNA Transfection Reagent
GeneJuice® Transfection Reagent
293-Free Transfection Protocol (pdf) NanoJuice Core Protocol (pdf) ProteoJuice Transfection Protocol (pdf) RiboJuice Transfection Protocol (pdf)GeneJuice Transfection Protcol (pdf)
OZbiosciences http://www.ozbiosciences.com/
DreamFect
DreamFect Gold
Lullaby
DreamFectTM protocolDreamFectTM Gold protocolLullabyTM protocol
PolyPlus Transfection http://www.polyplus-transfection.com/
INTERFERin® INTERFERin Protocol
Qiagen
HiPerfect
SuperFect
Effectine
RNAiFect
PolyFect
HiPerFect Protocol
SuperFect Protocol
Effectene Protocol
RNAiFect Protocol
PolyFect Protocol
Roche http://www.roche-applied-science.com/
X-tremeGENE siRNA Transfection Reagent
X-tremeGENE 9 Transfection Reagent
DOTAP Liposomal Transfection Reagent
X-tremeGENE ProtocolX-tremeGENE 9 ProtocolDOTAP Protocol
Sigma www.sigmaaldrich.com/life-science
EscortTM Transfection Reagent
EscortTM II Transfection Reagent
EscortTM III Transfection Reagent
EscortTM IV Transfection Reagent
Escort ProtocolEscort II ProtocolEscort III ProtocolEscort IV Protocol
SignaGen
PepMuteTM
PepMuteTM Plus
GenMuteTM
PowerFectTM
PepMute ProtocolPepMute Plus ProtocolGenMute ProtocolPowerFect Protocol

Useful Guides and References

J. Zabner, A.J. Fasbender, T. Moninger, K.A. Poellinger, M.J. Welsh; (1995) Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem. 270 (32), 18997 - 19007

Transfection Assay Troubleshooting

Transfection Reagent FAQ's