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There are a wide range of commercially available stains for High Content Imaging. Please contact the TDI to discuss the range of fluorophores available for your screening requirements. 

Invitrogen's Cell Staining Tool allows you to stain your own cell using their custom simulation tool to develop reproducible results with many of their fluorescent dyes.

Invitrogen Cell Staining Tool and Invitrogen Organelle Staining Guide.

This is a useful tool for assessing compatible flourophores.

Click on the Organelle you are interested in to see the range of stains available.

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Organelle Stains
Organelle - Adiposomes
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech LipidTOX™ Deep Red neutral lipid stain H34477 637/655 X X X
LipidTOX™ Green neutral lipid stain H34475 495/505 X X X
LipidTOX™ Red neutral lipid stain H34476 577/609 X X X
Organelle - Cytoplasm
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Calcein AM C3100MP 494/517 X    
CellTrace™ calcein red-orange AM C34851 576/589 X    
CellTrace™ Far Red DDAO-SE C34553 646/659 X    
Organelle Lights™ Cyto-GFP O36227 485/520 X   X
CellTracker™ Blue CMAC C2110 353/466 X    
CellTracker™ Green CMFDA C7025 492/517 X    
CellTracker™ Orange CMTMR C2927 541/565 X    
CellTracker™ Red CMTPX C34552 577/602 X    
Qtracker® 525 Cell Labeling Kit Q25041 535 X   X
Qtracker® 565 Cell Labeling Kit Q25031 565 X   X
Qtracker® 585 Cell Labeling Kit Q25001 585 X   X
Qtracker® 605 Cell Labeling Kit Q25011 605 X   X
Qtracker® 655 Cell Labeling Kit Q25021 655 X   X
Qtracker® 705 Cell Labeling Kit Q25061 705 X   X
Qtracker® 800 Cell Labeling Kit Q25071 800 X    
Organelle - Cytoskeleton
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Alexa Fluor® 350 phalloidin A22281 346/443   X X
Alexa Fluor® 488 phalloidin A12379 495/519   X X
Alexa Fluor® 555 phalloidin A34055 555/565   X X
Alexa Fluor® 594 phalloidin A12381 581/609   X X
Alexa Fluor® 635 phalloidin A34054 633/647   X X
Alexa Fluor® 647 phalloidin A22286 650/688   
Alexa Fluor® 680 phalloidin A22287 679/702   X X
CellLight™ Actin-GFP C10582 488/5510 X   X
Cellular Lights™ Actin-RFP C10127 555/584 X   X
CellLight™ Tubulin-GFP C10613 488/510 X   X
CellLight™ Tubulin-RFP C10614 555/584 X   X
CellLight™ MAP4-GFP C10598 488/510 X   X
CellLight™ MAP4-RFP C10599 555/584 X   X
Rhodamine phalloidin R415 540/565 X X  
TubulinTracker™ Green T34075 494/522 X    
New England Biolabs Alexa Fluor® 647 Phalloidin 8940 650/688   X X
Alexa Fluor® 488 Phalloidin 8878 495/519   X X
Alexa Fluor® 555 Phalloidin 8953 555/565   X X
Organelle - Endoplasmic reticulum
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Concanavalin A, Alexa Fluor® 594 conjugate C11253 590/617   X  
ER-Tracker™ Green E34251 540/511 X   X
ER-Tracker™ Red E34250 587/625 X   X
CellLight™ ER-GFP C10590 485/510 X   X
CellLight™ ER-RFP C10591 555/585 X   X
SelectFX® Alexa Fluor® 488 ER Labeling Kit S34200 495/519   X  
New England Biolabs ER-Tracker™ Green (BODIPY® FL Glibenclamide) 8787 540/511 X   X
Organelle - Golgi complex
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech BODIPY® FL C5-ceramide complexed to BSA D3521 505/511 X   X
B22650 505/511 X   X
BODIPY® TR ceramide complexed to BSA D7540, 589/617 X   X
B34400 589/617 X   X
Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 488 conjugate L11271 495/519   X  
Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 647 conjugate L32454 650/668   X  
NBD C6-ceramide N1154 466/530      
CellLight™ Golgi-GFP C10592 488/510 X   X (paraformaldehyde)
CellLight™ Golgi-RFP C10593 555/584 X   X (paraformaldehyde)
Intracellular Membranes
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech CellTrace™ BODIPY® TR C34556 598/625 X X X
Image-IT® LIVE Intracellular Membrane and Nuclear Labeling Kit I34407 350/461 and 598/625 X X X
Organelle - Lysosomes
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Image-iT® LIVE Lysosomal and Nuclear Labeling Kit I34202 350/461 X    
    577/590 X    
LysoTracker® Blue DND-22 L7525 373/422 X    
LysoTracker® Green DND-26 L7526 504/511 X    
LysoTracker® Red DND-99 L7528 577/590 X    
LysoSensor™ Blue DND-167 L7533 373/425 X    
LysoSensor™ Green DND-153 L7534 442/5005 X    
LysoSensor™ Yellow/Blue dextran, 10,000 MW L22460 335/381 and 425/521 X    
LysoSensor™ Yellow/Blue DND-160 L7545 328/384 and 440/540 X    
CellLight™ Lysosomes- C10596 488/510 X    
CellLight™ Lysosomes-RFP C10597 555/584 X    
New England Biolabs LysoTracker® Green DND-26 8783 442/5005 X    
Membrane Trafficking
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech CellLight™ Early Endosomes-GFP C10586 488/510 X   X (paraformaldehyde)
CellLight™ Early Endosomes-RFP C10587 555/584 X   X (paraformaldehyde)
Organelle Lights™ Synaptophysin-GFP C10080 488/510 X   X (paraformaldehyde)
CellLight™ Synaptophysin-RFP C10610 555/584 X   X (paraformaldehyde)
Organelle - Mitochondria
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Anti-OxPhos Complex V d subunit antibody (detection with an Alexa Fluor® dye–labeled secondary antibody) 459000     X  
mage-iT® LIVE Mitochondrial and Nuclear Labeling Ki I34154 350/461 ; Hoechst 33342; 579/599, Mitotracker Red X   X
JC-1 T3168 514/529 monomer; 485-589/590 J-aggregate form) X    
MitoTracker® Green FM M7514 490/516 X    
MitoTracker® Red FM M22425 581/644 X    
MitoTracker® Orange CMTMRos M7510 544/576 X   X
MitoTracker® Red CMXRos M7512 579/599 X   X
MitoTracker® Deep Red FM M22426 644/656 X   X
MitoTracker® Orange CM-H2TMRos M7511 554/576 X   X
MitoTracker® Red CM-H2XRos M7513 597/599 X   X
CellLight™ Mitochondria-GFP C10600 485/510 X   X (paraformaldehyde)
CellLight™ Mitochondria-RFP C10601 555/584 X   X (paraformaldehyde)
New England Biolabs MitoTracker® Green FM 9074 490/516 X   X
MitoTracker® Red CMXRos 9082 579/599 X   X
MitoTracker® Deep Red FM 8778 644/656 X   X
Organelle - Peroxisomes
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech CellLight™ Peroxisome-GFP C10604 488/510 X    
SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit S34201 495/519   X X (paraformaldehyde)
Organelle - Plasma membrane
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Alexa Fluor® 350 WGA W11263 346/442 X X X
Alexa Fluor® 488 WGA W11261 495/519 X X X
Alexa Fluor® 555 WGA W32464 555/565 X X X
Alexa Fluor® 594 WGA W11262 590/617 X X X
Alexa Fluor® 633 WGA W21404 632/647 X X X
Alexa Fluor® 647 WGA W32466 650/665 X X X
Alexa Fluor® 680 WGA W32465 679/702 X X X
CellTracker™ CM-DiI C7001 553/570 X   X
FM® 1-43FX F35355 510/626 X   X
FM® 4-64FX F34653 565/744 X   X
Image-iT® LIVE Plasma Membrane and Nuclear Labeling Kit I34406 350/461 (Hoechst 33342); 590/617 AlexaFluor 594 WGA) X X X
Lipophilic Tracer Sampler Kit (DiI, DiO, DiR, DiD, DiA) L7781 Several X X X
CellLight™ Plasma Membrane-CFP C10606 440/480 X   X (paraformaldehyde)
CellLight™ Plasma Membrane-GFP C10607 488/510 X   X (paraformaldehyde)
CellLight™ Plasma Membrane-RFP C10608 555/584 X   X (paraformaldehyde)
Nucleus
Nucleus
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech 7-AAD A1310 546/647   X  
CellLight™ Histone 2B-GFP C10594 488/510 X   X
CellLight™ Histone 2B-RFP C10595 555/584 X   X
DAPI D1306 358/461 X X  
Ethidium homodimer 1 E1169 528/617   X  
Hoechst 33342 H1399, H3570 350/461 X X  
Organelle Lights™ Nuc-CFP O36218 435/485 X   X (paraformaldehyde)
Organelle Lights™ Nuc-GFP O36209 485/520 X   X (paraformaldehyde)
CellLight™ Nucleus-GFP C10602 488/510 X   X
CellLight™ Nucleus-RFP C10603 555/584 X   X
Propidium iodide P3566 530/625   X  
SelectFX® Nuclear Labeling Kit, for fixed cells (DAPI, SYTOX® Green, 7-AAD, TO-PRO®-3 iodide) S33025 Several   X  
SYTO® 9 green fluorescent nucleic acid stain S34854 483/503 X X  
SYTO® 14 green fluorescent nucleic acid stain S7576 517/549 (DNA) 521/547 (RNA) X X  
SYTO® 82 orange fluorescent nucleic acid stain S11363 541/560 X X  
SYTO® 59 red fluorescent nucleic acid stain S11341 622/645 X X  
SYTO® 61 red fluorescent nucleic acid stain S11343 628/645 X X  
SYTOX® Green nucleic acid stain S7020 504/523   X  
SYTOX® Orange nucleic acid stain S11368 547/570   X  
TO-PRO®-3 iodide T3605 642/661   X  
Thermo Scientific (Pierce) DAPI Nuclear Counter Stain 62247 358/461 X X  
DAPI Nuclear Counter Stain 62248 358/461 X X  
DAPI Nuclear Counter Stain 46190 358/461 X X  
DRAQ-5 Far Red DNA Stain 62254 646/697 X X  
DRAQ-5 Far Red DNA Stain 62251 646/697 X X  
DRAQ-5 Far Red DNA Stain 62252 646/697 X X  
Hoechst 33342 62249 343/497 X X  
New England Biolabs DAPI 4083 358/461 X X  
DRAQ5® 4084 646/697      
DRAQ7™ 7406 646/697      
Hoechst 33342 4082 350/461 X X  
Nucleus - Nuclear envelope
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech Organelle Lights™ NF GFP O36213 485/520 X X (paraformaldehyde)  
Nucleus - Nucleoli
Supplier Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech SYTO® 81 Orange nucleic acid stain S11362 530/544 X   X
SYTO® RNASelect™ Green S32703 490/530 (bound to RNA) X   X
Whole-cell stains for image segmentation in HCS applications
Supplier  Product Name Catalogue Number Excitation (nm) Live Cell Fixed Cell Fixable
LifeTech CellMask™ Blue stain H34558 346/442   X  
CellMask™ Deep Red stain H34560 650/665   X  
CellMask™ Red stain H32711 622/645   X  
Thermo Scientific (Pierce) Whole Cell Stain Blue 8403501 350/450   X  
Whole Cell Stain Blue 8403502 350/450   X  
Whole Cell Stain Green 8403201 493/518   X  
Whole Cell Stain Green  8403202 493/518   X  
Whole Cell Stain Orange 8403301 550/568   X  
Whole Cell Stain Orange 8403302 550/568   X  
Whole Cell Stain Red 8403401 654/673   X  
Whole Cell Stain Red 8403402 654/673   X