High Content Imaging Stains

There are a wide range of commercially available stains for High Content Imaging. Please contact the TDI to discuss the range of fluorophores available for your screening requirements.

Invitrogen's Cell Staining Tool allows you to stain your own cell using their custom simulation tool to develop reproducible results with many of their fluorescent dyes.

Invitrogen Cell Staining Tool and Invitrogen Organelle Staining Guide.

This is a useful tool for assessing compatible flourophores.

Click on the Organelle you are interested in to see the range of stains available.

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Organelle Stains
Organelle - Adiposomes
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	LipidTOX™ Deep Red neutral lipid stain	H34477	637/655	X	X	X
	LipidTOX™ Green neutral lipid stain	H34475	495/505	X	X	X
	LipidTOX™ Red neutral lipid stain	H34476	577/609	X	X	X
Organelle - Cytoplasm
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Calcein AM	C3100MP	494/517	X
	CellTrace™ calcein red-orange AM	C34851	576/589	X
	CellTrace™ Far Red DDAO-SE	C34553	646/659	X
	Organelle Lights™ Cyto-GFP	O36227	485/520	X		X
	CellTracker™ Blue CMAC	C2110	353/466	X
	CellTracker™ Green CMFDA	C7025	492/517	X
	CellTracker™ Orange CMTMR	C2927	541/565	X
	CellTracker™ Red CMTPX	C34552	577/602	X
	Qtracker® 525 Cell Labeling Kit	Q25041	535	X		X
	Qtracker® 565 Cell Labeling Kit	Q25031	565	X		X
	Qtracker® 585 Cell Labeling Kit	Q25001	585	X		X
	Qtracker® 605 Cell Labeling Kit	Q25011	605	X		X
	Qtracker® 655 Cell Labeling Kit	Q25021	655	X		X
	Qtracker® 705 Cell Labeling Kit	Q25061	705	X		X
	Qtracker® 800 Cell Labeling Kit	Q25071	800	X
Organelle - Cytoskeleton
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Alexa Fluor® 350 phalloidin	A22281	346/443		X	X
	Alexa Fluor® 488 phalloidin	A12379	495/519		X	X
	Alexa Fluor® 555 phalloidin	A34055	555/565		X	X
	Alexa Fluor® 594 phalloidin	A12381	581/609		X	X
	Alexa Fluor® 635 phalloidin	A34054	633/647		X	X
	Alexa Fluor® 647 phalloidin	A22286	650/688		X	X
	Alexa Fluor® 680 phalloidin	A22287	679/702		X	X
	CellLight™ Actin-GFP	C10582	488/5510	X		X
	Cellular Lights™ Actin-RFP	C10127	555/584	X		X
	CellLight™ Tubulin-GFP	C10613	488/510	X		X
	CellLight™ Tubulin-RFP	C10614	555/584	X		X
	CellLight™ MAP4-GFP	C10598	488/510	X		X
	CellLight™ MAP4-RFP	C10599	555/584	X		X
	Rhodamine phalloidin	R415	540/565	X	X
	TubulinTracker™ Green	T34075	494/522	X
New England Biolabs	Alexa Fluor® 647 Phalloidin	8940	650/688		X	X
	Alexa Fluor® 488 Phalloidin	8878	495/519		X	X
	Alexa Fluor® 555 Phalloidin	8953	555/565		X	X
Organelle - Endoplasmic reticulum
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Concanavalin A, Alexa Fluor® 594 conjugate	C11253	590/617		X
	ER-Tracker™ Green	E34251	540/511	X		X
	ER-Tracker™ Red	E34250	587/625	X		X
	CellLight™ ER-GFP	C10590	485/510	X		X
	CellLight™ ER-RFP	C10591	555/585	X		X
	SelectFX® Alexa Fluor® 488 ER Labeling Kit	S34200	495/519		X
New England Biolabs	ER-Tracker™ Green (BODIPY® FL Glibenclamide)	8787	540/511	X		X
Organelle - Golgi complex
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	BODIPY® FL C5-ceramide complexed to BSA	D3521	505/511	X		X
	BODIPY® FL C5-ceramide complexed to BSA	B22650	505/511	X		X
	BODIPY® TR ceramide complexed to BSA	D7540,	589/617	X		X
	BODIPY® TR ceramide complexed to BSA	B34400	589/617	X		X
	Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 488 conjugate	L11271	495/519		X
	Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 647 conjugate	L32454	650/668		X
	NBD C6-ceramide	N1154	466/530
	CellLight™ Golgi-GFP	C10592	488/510	X		X (paraformaldehyde)
	CellLight™ Golgi-RFP	C10593	555/584	X		X (paraformaldehyde)
Intracellular Membranes
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	CellTrace™ BODIPY® TR	C34556	598/625	X	X	X
LifeTech	Image-IT® LIVE Intracellular Membrane and Nuclear Labeling Kit	I34407	350/461 and 598/625	X	X	X
Organelle - Lysosomes
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Image-iT® LIVE Lysosomal and Nuclear Labeling Kit	I34202	350/461	X
			577/590	X
	LysoTracker® Blue DND-22	L7525	373/422	X
	LysoTracker® Green DND-26	L7526	504/511	X
	LysoTracker® Red DND-99	L7528	577/590	X
	LysoSensor™ Blue DND-167	L7533	373/425	X
	LysoSensor™ Green DND-153	L7534	442/5005	X
	LysoSensor™ Yellow/Blue dextran, 10,000 MW	L22460	335/381 and 425/521	X
	LysoSensor™ Yellow/Blue DND-160	L7545	328/384 and 440/540	X
	CellLight™ Lysosomes-	C10596	488/510	X
	CellLight™ Lysosomes-RFP	C10597	555/584	X
New England Biolabs	LysoTracker® Green DND-26	8783	442/5005	X
Membrane Trafficking
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	CellLight™ Early Endosomes-GFP	C10586	488/510	X		X (paraformaldehyde)
	CellLight™ Early Endosomes-RFP	C10587	555/584	X		X (paraformaldehyde)
	Organelle Lights™ Synaptophysin-GFP	C10080	488/510	X		X (paraformaldehyde)
	CellLight™ Synaptophysin-RFP	C10610	555/584	X		X (paraformaldehyde)
Organelle - Mitochondria
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Anti-OxPhos Complex V d subunit antibody (detection with an Alexa Fluor® dye–labeled secondary antibody)	459000			X
	mage-iT® LIVE Mitochondrial and Nuclear Labeling Ki	I34154	350/461 ; Hoechst 33342; 579/599, Mitotracker Red	X		X
	JC-1	T3168	514/529 monomer; 485-589/590 J-aggregate form)	X
	MitoTracker® Green FM	M7514	490/516	X
	MitoTracker® Red FM	M22425	581/644	X
	MitoTracker® Orange CMTMRos	M7510	544/576	X		X
	MitoTracker® Red CMXRos	M7512	579/599	X		X
	MitoTracker® Deep Red FM	M22426	644/656	X		X
	MitoTracker® Orange CM-H2TMRos	M7511	554/576	X		X
	MitoTracker® Red CM-H2XRos	M7513	597/599	X		X
	CellLight™ Mitochondria-GFP	C10600	485/510	X		X (paraformaldehyde)
	CellLight™ Mitochondria-RFP	C10601	555/584	X		X (paraformaldehyde)
New England Biolabs	MitoTracker® Green FM	9074	490/516	X		X
	MitoTracker® Red CMXRos	9082	579/599	X		X
	MitoTracker® Deep Red FM	8778	644/656	X		X
Organelle - Peroxisomes
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	CellLight™ Peroxisome-GFP	C10604	488/510	X
LifeTech	SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit	S34201	495/519		X	X (paraformaldehyde)
Organelle - Plasma membrane
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Alexa Fluor® 350 WGA	W11263	346/442	X	X	X
	Alexa Fluor® 488 WGA	W11261	495/519	X	X	X
	Alexa Fluor® 555 WGA	W32464	555/565	X	X	X
	Alexa Fluor® 594 WGA	W11262	590/617	X	X	X
	Alexa Fluor® 633 WGA	W21404	632/647	X	X	X
	Alexa Fluor® 647 WGA	W32466	650/665	X	X	X
	Alexa Fluor® 680 WGA	W32465	679/702	X	X	X
	CellTracker™ CM-DiI	C7001	553/570	X		X
	FM® 1-43FX	F35355	510/626	X		X
	FM® 4-64FX	F34653	565/744	X		X
	Image-iT® LIVE Plasma Membrane and Nuclear Labeling Kit	I34406	350/461 (Hoechst 33342); 590/617 AlexaFluor 594 WGA)	X	X	X
	Lipophilic Tracer Sampler Kit (DiI, DiO, DiR, DiD, DiA)	L7781	Several	X	X	X
	CellLight™ Plasma Membrane-CFP	C10606	440/480	X		X (paraformaldehyde)
	CellLight™ Plasma Membrane-GFP	C10607	488/510	X		X (paraformaldehyde)
	CellLight™ Plasma Membrane-RFP	C10608	555/584	X		X (paraformaldehyde)
Nucleus
Nucleus
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	7-AAD	A1310	546/647		X
	CellLight™ Histone 2B-GFP	C10594	488/510	X		X
	CellLight™ Histone 2B-RFP	C10595	555/584	X		X
	DAPI	D1306	358/461	X	X
	Ethidium homodimer 1	E1169	528/617		X
	Hoechst 33342	H1399, H3570	350/461	X	X
	Organelle Lights™ Nuc-CFP	O36218	435/485	X		X (paraformaldehyde)
	Organelle Lights™ Nuc-GFP	O36209	485/520	X		X (paraformaldehyde)
	CellLight™ Nucleus-GFP	C10602	488/510	X		X
	CellLight™ Nucleus-RFP	C10603	555/584	X		X
	Propidium iodide	P3566	530/625		X
	SelectFX® Nuclear Labeling Kit, for fixed cells (DAPI, SYTOX® Green, 7-AAD, TO-PRO®-3 iodide)	S33025	Several		X
	SYTO® 9 green fluorescent nucleic acid stain	S34854	483/503	X	X
	SYTO® 14 green fluorescent nucleic acid stain	S7576	517/549 (DNA) 521/547 (RNA)	X	X
	SYTO® 82 orange fluorescent nucleic acid stain	S11363	541/560	X	X
	SYTO® 59 red fluorescent nucleic acid stain	S11341	622/645	X	X
	SYTO® 61 red fluorescent nucleic acid stain	S11343	628/645	X	X
	SYTOX® Green nucleic acid stain	S7020	504/523		X
	SYTOX® Orange nucleic acid stain	S11368	547/570		X
	TO-PRO®-3 iodide	T3605	642/661		X
Thermo Scientific (Pierce)	DAPI Nuclear Counter Stain	62247	358/461	X	X
	DAPI Nuclear Counter Stain	62248	358/461	X	X
	DAPI Nuclear Counter Stain	46190	358/461	X	X
	DRAQ-5 Far Red DNA Stain	62254	646/697	X	X
	DRAQ-5 Far Red DNA Stain	62251	646/697	X	X
	DRAQ-5 Far Red DNA Stain	62252	646/697	X	X
	Hoechst 33342	62249	343/497	X	X
New England Biolabs	DAPI	4083	358/461	X	X
	DRAQ5®	4084	646/697
	DRAQ7™	7406	646/697
	Hoechst 33342	4082	350/461	X	X
Nucleus - Nuclear envelope
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	Organelle Lights™ NF GFP	O36213	485/520	X	X (paraformaldehyde)
Nucleus - Nucleoli
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	SYTO® 81 Orange nucleic acid stain	S11362	530/544	X		X
LifeTech	SYTO® RNASelect™ Green	S32703	490/530 (bound to RNA)	X		X
Whole-cell stains for image segmentation in HCS applications
Supplier	Product Name	Catalogue Number	Excitation (nm)	Live Cell	Fixed Cell	Fixable
LifeTech	CellMask™ Blue stain	H34558	346/442		X
	CellMask™ Deep Red stain	H34560	650/665		X
	CellMask™ Red stain	H32711	622/645		X
Thermo Scientific (Pierce)	Whole Cell Stain Blue	8403501	350/450		X
	Whole Cell Stain Blue	8403502	350/450		X
	Whole Cell Stain Green	8403201	493/518		X
	Whole Cell Stain Green	8403202	493/518		X
	Whole Cell Stain Orange	8403301	550/568		X
	Whole Cell Stain Orange	8403302	550/568		X
	Whole Cell Stain Red	8403401	654/673		X
	Whole Cell Stain Red	8403402	654/673		X

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High Content Imaging Stains